國家衛生研究院 NHRI:Item 3990099045/14946

NHRI > National Institute of Environmental Health Sciences > Po-Chin Huang > Periodical Articles > Item 3990099045/14946

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14946

Title:	Ultra-sensitive determination of Ochratoxin A in coffee and tea samples using a novel semi-automated in-syringe based coagulant-assisted fast mycotoxin extraction (FaMEx) technique coupled with UHPLC-MS/MS
Authors:	Prakasham, K;Gurrani, S;Shiea, J;Wu, MT;Wu, CF;Lin, YC;Tsai, B;Huang, PC;Andaluri, G;Ponnusamy, VK
Contributors:	National Institute of Environmental Health Sciences
Abstract:	In this study, we demonstrated a novel semi-automated in-syringe-based coagulant-assisted liquid–liquid microextraction (IS-CGA-LLME) as fast mycotoxin extraction (FaMEx) technique coupled with ultra-high-performance liquid chromatography connected with a tandem-mass spectrometer (UHPLC-MS/MS) for the quantification of mycotoxin (Ochratoxin A, OT-A) in coffee and tea samples. IS-CGA-LLME is a three-step extraction process that includes extraction of OT-A from sample matrix using low-volume solvent extraction, then the extractant was cleaned-up using a coagulation process, and finally, the decolorized/matrix removed sample solution was processed for LLME for target analyte's pre-concentration. The final extractant was analyzed using UHPLC-MS/MS for OT-A quantification. Under the optimized experimental conditions, highly sensitive detection and quantification limits were obtained at 0.001 and 0.003 ng g−1 for OT-A with excellent extraction recovery (93–111%) and precision <10%. These results proved that the developed method is a simple, highly sensitive, semi-automated, low-matrix effect and efficient procedure for the determination of mycotoxins in food samples.
Date:	2023-08-15
Relation:	Food Chemistry. 2023 Aug 15;417:Article number 135951.
Link to:	http://dx.doi.org/10.1016/j.foodchem.2023.135951
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0308-8146&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000957835100001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85150258217
Appears in Collections:	[Po-Chin Huang] Periodical Articles

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