國家衛生研究院 NHRI:Item 3990099045/14960

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14960

Title:	Hippo signaling pathway contributes to air pollution exposure-induced emphysema in ageing rats
Authors:	Laiman, V;Hsiao, TC;Fang, YT;Chen, YY;Lo, YC;Lee, KY;Chen, TT;Chen, KY;Ho, SC;Wu, SM;Chen, JK;Heriyanto, DS;Chung, KF;Ho, KF;Chuang, KJ;Chang, JH;Chuang, HC
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	We investigated Hippo signaling pathway components in emphysema development of air pollutionexposed ageing rats. Male 1.5-year-old Fischer 344 ageing rats were exposed to low-level trafficrelated air pollution via whole-body exposure for 3 months with/without highefficiency particulate air (HEPA) filtration (gaseous vs. gaseous and particulate matter with aerodynamic diameter of ≤ 2.5 µ m (PM 2.5 )). MLE-12 type II alveolar epithelial cells (AECII) were exposed to 0, 1, and 50 μ g/mL diesel exhaust particles (DEPs). Human lung sections from non-smokers, smokers, and chronic obstructive pulmonary disease (COPD) subjects were used for confirmation. RNA-sequencing revealed that DEP decreased lung development-related genes. Air pollution resulted in 52.05% PM 2.5 being deposited in alveolar region, with emphysema and increased airway thickness by micro-computed tomography (CT) and histological sections. Air pollution decreased phosphorylated (p)YAP/YAP ratio, while increasing pTAZ/TAZ ratio in DEP-exposed MLE-12 AECII and in rat SPC-positive (SPC þ ) AECII. Air pollution decreased E-cadherin and ⍺ -catenin, while increasing SPC, LGALS3, and T1 ⍺ expressions. Air pollution decreased lung pSIRT1/SIRT1 ratio. In human subjects, smokers SPC þ AECII showed decreased pYAP/ YAP ratio. In conclusion, air pollution can cause emphysema by Hippo pathway dysregulation with decreased cell adherens junctions, increased cell differentiation, and senescence. Hippo pathway dysregulation may also cause emphysema development in smokers. Graphical abstract.
Date:	2023-06-15
Relation:	Journal of Hazardous Materials. 2023 Jun 15;452:Article number 131188.
Link to:	http://dx.doi.org/10.1016/j.jhazmat.2023.131188
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0304-3894&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000961368500001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85159243993
Appears in Collections:	[Jen-Kun Chen] Periodical Articles

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