國家衛生研究院 NHRI:Item 3990099045/15006

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Title:	3D-cultured adipose-derived stem cell spheres using calcium-alginate scaffolds for osteoarthritis treatment in a mono-iodoacetate-induced rat model
Authors:	Lin, YY;Kuan, CY;Chang, CT;Chuang, MH;Syu, WS;Zhang, KL;Lee, CH;Lin, PC;Dong, GC;Lin, FH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Osteoarthritis (OA) is a degenerative disease that causes pain, cartilage deformation, and joint inflammation. Mesenchymal stem cells (MSCs) are potential therapeutic agents for OA treatment. However, the 2D culture of MSCs could potentially affect their characteristics and functionality. In this study, calcium-alginate (Ca-Ag) scaffolds were prepared for human adipose-derived stem cell (hADSC) proliferation with a homemade functionally closed process bioreactor system; the feasibility of cultured hADSC spheres in heterologous stem cell therapy for OA treatment was then evaluated. hADSC spheres were collected from Ca-Ag scaffolds by removing calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation. In this study, 2D-cultured individual hADSCs or hADSC spheres were evaluated for treatment efficacy in a monosodium iodoacetate (MIA)-induced OA rat model. The results of gait analysis and histological sectioning showed that hADSC spheres were more effective at relieving arthritis degeneration. The results of serological and blood element analyses of hADSC-treated rats indicated that the hADSC spheres were a safe treatment in vivo. This study demonstrates that hADSC spheres are a promising treatment for OA and can be applied to other stem cell therapies or regenerative medical treatments.
Date:	2023-04-11
Relation:	International Journal of Molecular Sciences. 2023 Apr 11;24(8):Article number 7062.
Link to:	http://dx.doi.org/10.3390/ijms24087062
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1422-0067&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000979381800001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85156269707
Appears in Collections:	[Feng-Huei Lin] Periodical Articles [Guo-Chung Dong] Periodical Articles

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