國家衛生研究院 NHRI:Item 3990099045/15021

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Title:	Dual-well microfluidic technique for single cell isolation and long-term clonal culture
Authors:	Yeh, CF;Chang, HC;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	The clonal culture of single cells is widely used for studying cell dynamics and heterogeneity in vitro and producing monoclonal cell populations. Using traditional culture systems such as well plates to perform single cell culture experiments is of low efficiency because the Poisson distribution limits the available highest single cell event probability. Here we describe high-efficiency single cell isolation and long-term culture technique, which is based on using a microfluidic chip with a dual-well microchannel design. The utility of this technique is demonstrated with the isolation and culture of cancer and stem cells.
Date:	2021-10-29
Relation:	Handbook of Single-Cell Technologies. 2021 Oct 29:263-286.
Link to:	http://dx.doi.org/10.1007/978-981-10-8953-4_26
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85159412217
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles

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