國家衛生研究院 NHRI:Item 3990099045/15028

NHRI > National Institute of Cancer Research > Ming Jacqueline Liu(1996-2007) > Periodical Articles > Item 3990099045/15028

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15028

Title:	Generation and characterization of avian single chain variable fragment against human Alpha-Enolase
Authors:	Lee, CH;Tsai, CH;Leu, SJ;Liu, KJ;Wang, WC;Tsai, BY;Chiang, LC;Mao, YC;Benedict Dlamini, N;Tsai, CH;Yang, YY
Contributors:	National Institute of Cancer Research
Abstract:	Overexpression of human alpha-enolase (hEno1) has been reported in a wide range of cancers and is tightly associated with poor prognosis, making it a remarkable biomarker and therapeutic target. In this study, polyclonal yolk-immunoglobulin (IgY) antibodies purified from hEno1-immunized chickens showed a noticeable specific humoral response. Phage display technology was used to construct two antibody libraries of IgY gene-derived single-chain variable fragments (scFvs) containing 7.8 × 107 and 5.4 × 107 transformants, respectively. Phage-based ELISA indicated that specific anti-hEno1 clones were significantly enriched. The nucleotide sequences of scFv-expressing clones were determined and classified into seven groups either in the short linker or the long linker. Moreover, higher mutation rates were revealed in the CDR regions, especially in the CDR3. Three distinguish antigenic epitopes were identified on the hEno1 protein. The binding activities of selected anti-hEno1 scFv on hEno1-positive PE089 lung cancer cells were confirmed using Western blot, flow cytometry, and immunofluorescence assay. In particular, hEnS7 and hEnS8 scFv antibodies significantly suppressed the growth and migration of PE089 cells. Taken together, these chicken-derived anti-hEno1 IgY and scFv antibodies have great potential to develop diagnostic and therapeutic agents for the treatment of lung cancer patients with high expression levels of hEno1 protein.
Date:	2023-07
Relation:	International Immunopharmacology. 2023 Jul;120:Article number 110277.
Link to:	http://dx.doi.org/10.1016/j.intimp.2023.110277
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1567-5769&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001002058300001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85159455502
Appears in Collections:	[Ming Jacqueline Liu(1996-2007)] Periodical Articles

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