國家衛生研究院 NHRI:Item 3990099045/15658

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15658

Title:	Microbiota signatures associated with invasive Candida albicans infection in the gastrointestinal tract of immunodeficient mice
Authors:	Yan, JY;Lin, TH;Jong, YT;Hsueh, JW;Wu, SH;Lo, HJ;Chen, YC;Pan, CH
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	Candida albicans is a commensal microorganism in the human gut but occasionally causes invasive C. albicans infection (ICA), especially in immunocompromised individuals. Early initiation of antifungal therapy is associated with reduced mortality of ICA, but rapid diagnosis remains a challenge. The ICA-associated changes in the gut microbiota can be used as diagnostic and therapeutic targets but have been poorly investigated. In this study, we utilized an immunodeficient Rag2 gamma c (Rag2-/-il2 gamma c-/-) mouse model to investigate the gut microbiota alterations caused by C. albicans throughout its cycle, from its introduction into the gastrointestinal tract to invasion, in the absence of antibiotics. We observed a significant increase in the abundance of Firmicutes, particularly Lachnospiraceae and Ruminococcaceae, as well as a significant decrease in the abundance of Candidatus Arthromitus in mice exposed to either the wild-type SC5314 strain or the filamentation-defective mutant (cph1/cph1 efg1/efg1) HLC54 strain of C. albicans. However, only the SC5314-infected mice developed ICA. A linear discriminate analysis of the temporal changes in the gut bacterial composition revealed Bacteroides vulgatus as a discriminative biomarker associated with SC5314-infected mice with ICA. Additionally, a positive correlation between the B. vulgatus abundance and fungal load was found, and the negative correlation between the Candidatus Arthromitus abundance and fungal load after exposure to C. albicans suggested that C. albicans might affect the differentiation of intestinal Th17 cells. Our findings reveal the influence of pathogenic C. albicans on the gut microbiota and identify the abundance of B. vulgatus as a microbiota signature associated with ICA in an immunodeficient mouse model.
Date:	2024-01-17
Relation:	Frontiers in Cellular and Infection Microbiology. 2024 Jan 17;13:Article number 1278600.
Link to:	http://dx.doi.org/10.3389/fcimb.2023.1278600
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2235-2988&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001154116000001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85183609916
Appears in Collections:	[Chien-Hsiung Pan] Periodical Articles [Yee-Chun Chen] Periodical Articles [Hsiu-Jung Lo] Periodical Articles

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