A uniform dispersion of TiO2/SiO2 hollow nanosphere of 290 nm with a high surface area of more than 100 m(2)g(- 1) is prepared using polystyrene spheres as templates after calcination at 600 degrees C. The TiO2/SiO2 hollow nanospheres are characterized by scanning electron microscopy, transmission electron microscopy, dynamic light scattering, nitrogen adsorption-desorption isotherms, and X-ray diffraction. The TiO2/SiO2 hollow nanospheres are shown to be highly effective in selectively enriching phosphopeptides, resulting in a significant reduction of non-phosphorylated species and an enhanced presence of modified peptides. The favorable results obtained in the present study for the enrichment of phosphoprotein beta-casein pave the way toward a similar treatment of other types of biomolecules.
Date:
2024-02-10
Relation:
Journal of Porous Materials. 2024 Feb 10;Article in Press.
