一种于大肠杆菌制造重组脂质化多肽的方法。该方法包括：提供一种经适应表现重组脂质化多肽的大肠杆菌宿主细胞；以及将该大肠杆菌宿主细胞在能表现呈脂质化形式的多肽的条件下，培养于基本培养基(minimal？medium)中。A method of producing a recombinant lipidated polypeptide in E. coli. The method includes providing an E. coli host cell adapted to express a recombinant lipidated polypeptide; and culturing the E. coli host cell in a minimal medium under conditions that allow expression of the polypeptide in lipidated form.
