國家衛生研究院 NHRI:Item 3990099045/16364
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/16364


    题名: Antibodies and method for determining deletions in HBV pre-S2 region
    作者: Huang, WN;Su, IJ;Lee, YP
    贡献者: National Institute of Infectious Diseases and Vaccinology
    摘要: A HBS-specific antibody, a LHBS-specific antibody, a WT LHBS-specific antibody, an immunoassay kit comprising the antibodies, and a method of detecting pre-S2 deletion mutant LHBS using the immunoassay kit are disclosed herein. The method comprises incubating a biological sample with a first antibody to captured HBS proteins; detecting the LHBS and WT LHBS bound to the immobilized first antibody, respectively; and calculating the amount of the pre-S2 deletion mutant LHBS protein by subtracting the amount of the WT LHBS protein from that of the LHBS protein. Advantageously, by the method described herein, the amount of the pre-S2 deletion mutant LHBS, a potential high-risk marker for HCC incidence in chronic HBV carriers and recurrence in HCC patients after hepatectomy surgery, in a biological sample may be easily calculated without mutual influence between the WT and pre-S mutant LHBS while reducing the labor-intensive process for cloning each gene product before analysis.
    日期: 2017-07-25
    關聯: US9714284/2017-07-25
    Link to: https://tiponet.tipo.gov.tw/gpss2/gpsskmc/gpssbkm?!!FRURLUS09714284B2
    显示于类别:[蘇益仁(2002-2015)] 專利

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