國家衛生研究院 NHRI:Item 3990099045/16392
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    題名: Microfluidic dual-well device for highthroughput single-cell capture and culture
    作者: Hsu, CH;Lin, CH;Chang, HC;Chiu, IM
    貢獻者: Institute of Biomedical Engineering and Nanomedicine;Institute of Cellular and Systems Medicine
    摘要: A microfluidic dual-well device is disclosed. The device comprises: (a) a first substrate having a first end, a second end, and a culture microwell forming portion; (b) a plurality of culture microwells; (c) a second substrate having a first end, a second end, and a capture microwell forming portion, the two ends of the second substrate being respectively bounded to the two ends of the first substrate; (d) a plurality of capture microwells; (e) a microfluidic channel; (f) a microfluidic inlet port; and (g) a microfluidic outlet port; wherein the microfluidic channel is in fluidic connections with the culture microwells, the capture microwells, and the inlet and outlet ports. Methods of capturing and transferring a single cell or a single cell colony for culture, and method of transferring a target cell from a polydimethylsiloxane (PDMS) structure of culture microwells to a culture plate for culture are also disclosed.
    日期: 2018-06-19
    關聯: US10000732/2018-06-19
    Link to: https://tiponet.tipo.gov.tw/gpss2/gpsskmc/gpssbkm?!!FRURLUS10000732B2
    顯示於類別:[許佳賢] 專利
    [邱英明] 專利

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