國家衛生研究院 NHRI:Item 3990099045/16437
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/16437


    Title: Transplantation of exosomes derived from human wharton's jelly mesenchymal stromal cells enhances functional improvement in stroke rats
    Authors: Chiu, YS;Wu, KJ;Yu, SJ;Wu, KL;Hsieh, CY;Chou, YS;Chen, KY;Wang, YS;Bae, EK;Hung, TW;Lin, SH;Lin, CH;Hsu, SC;Wang, Y;Chen, YH
    Contributors: Center for Neuropsychiatric Research;National Institute of Infectious Diseases and Vaccinology
    Abstract: Cerebral ischemic stroke is a major cerebrovascular disease and the leading cause of adult disability. We and others previously demonstrated that transplantation of human Wharton's jelly mesenchymal stromal cells (WJ-MSCs) attenuated neuronal damage and promoted functional improvement in stroke animals. This study aimed to investigate the protective effects of human WJ-MSC exosome (Exo) transplant in cellular and rat models of cerebral stroke. Administration of Exo significantly antagonized glutamate-mediated neuronal loss and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-X nick end labeling (TUNEL) in rat primary cortical neuronal cultures. Adult male rats underwent a 60-min middle cerebral artery occlusion (MCAo); Exo or vehicle was injected through the tail vein 5-10 min after the MCAo. Two days later, the rats underwent a series of behavioral tests. Stroke rats receiving Exo developed a significant improvement in locomotor function and forelimb strength while reductions in body asymmetry and Bederson's neurological score. After the behavioral test, brain tissues were harvested for histological and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analyses. Animals receiving Exo had less infarction volume, measured by 2,3,5-triphenyl tetrazolium chloride (TTC) staining. Transplantation of Exo increased the expression of protective neurotrophic factors (BMP7, GDNF) and anti-apoptotic factors (Bcl2, Bcl-xL) in the ischemic brain. These findings suggest that early post-treatment with WJ-MSC Exo, given non-invasively through the vein, improved functional recovery and reduced brain damage in the stroke brain.
    Date: 2024-12-03
    Relation: Cell Transplantation. 2024 Dec 03;Article in Press.
    Link to: http://dx.doi.org/10.1177/09636897241296366
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:001368343000001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85211144077
    Appears in Collections:[Yun Wang ] Periodical Articles
    [Seong-Jin Yu] Periodical Articles
    [Shu-Ching Hsu] Periodical Articles

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