國家衛生研究院 NHRI:Item 3990099045/1686
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    题名: Inhibition of hepatitis C virus replication by arsenic trioxide
    作者: Hwang, DR;Tsai, YC;Lee, JC;Huang, KK;Lin, RK;Ho, CH;Chiou, JM;Lin, YT;Hsu, JTA;Yeh, CT
    贡献者: Division of Biostatistics and Bioinformatics;Division of Biotechnology and Pharmaceutical Research
    摘要: Hepatitis C virus (HCV) is a serious global problem, and present therapeutics are inadequate to cure HCV infection. In the present study, various antiviral assays show that As2O3 at submicromolar concentrations is capable of inhibiting HCV replication. The 50% effective concentration (EC50) of As2O3 required to inhibit HCV replication was 0.35 muM when it was determined by a reporter-based HCV replication assay, and the EC50 was below 0.2 muM when it was determined by quantitative reverse transcription-PCR analysis. As2O3 did not cause cellular toxicity at this concentration, as revealed by an MTS [3-(4,5-dimethylthiozol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assay. A combination of As2O3 and alpha interferon exerted synergistic effects against HCV, as revealed by a multiple linear logistic model and isobolograrn analysis. Furthermore, in an alternative HCV antiviral system that may recapitulate additional steps involved in HCV infection and replication, As2O3 at 0.3 muM totally abolished the HCV signal, whereas alpha interferon at a high dose (5,000 IU/ml) only partially suppressed the HCV signal. The study highlights the indications for use of a novel class of anti-HCV agent. Further elucidation of the exact antiviral mechanism of As2O3 may lead to the development of agents with potent activities against HCV or related viruses.
    关键词: Microbiology;Pharmacology & Pharmacy
    日期: 2004-08
    關聯: Antimicrobial Agents and Chemotherapy. 2004 Aug;48(8):2876-2882.
    Link to: http://dx.doi.org/10.1128/AAC.48.8.2876-2882.2004
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000222998300014
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=3343022576
    显示于类别:[丘政民(2000-2004)] 期刊論文
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