國家衛生研究院 NHRI:Item 3990099045/16994
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/16994


    Title: PCK2 promotes invasion and epithelial-to-mesenchymal transition in triple-negative breast cancer by promoting TGF-beta/SMAD3 signaling through inhibiting TRIM67-mediated SMAD3 ubiquitination
    Other Titles: PCK2 promotes invasion and epithelial-to-mesenchymal transition in triple-negative breast cancer by promoting TGF-β/SMAD3 signaling through inhibiting TRIM67-mediated SMAD3 ubiquitination
    Authors: Chang, TM;Fang, WY;Hsu, HP;Chu, PY;Jiang, SS;Huang, KW;Hung, WC;Lin, HY;Tsai, HJ
    Contributors: National Institute of Cancer Research
    Abstract: PCK2, which encodes mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M), is upregulated in various cancers. We demonstrated high expression of PEPCK-M in approximately half of triple-negative breast cancers (TNBCs) previously. TNBC is associated with an aggressive phenotype and a high metastasis rate. In this study, we investigated the role of PCK2 in TNBC. PCK2 knockdown suppressed proliferation and mTOR signaling in TNBC cells. In addition, cell invasion/migration ability and the expression of epithelial-to-mesenchymal transition (EMT) markers were positively correlated with PCK2 expression in TNBC cells via regulation of transforming growth factor-beta (TGF-beta)/SMAD3 signaling. SMAD3 was positively regulated by PCK2 in TNBC cells. Knockdown of SMAD3 in PCK2-overexpressing TNBC cells reduced the expression levels of EMT markers, Snail and Slug, and suppressed cell invasion/migration. In addition, PCK2 knockdown attenuated the stimulatory effect of TGF-beta on SMAD3 phosphorylation in TNBC cells. PEPCK-M promotes the protein and mRNA expression of SMAD3 via competitive binding to tripartite motif-containing 67 (TRIM67), an E3 ubiquitin ligase, to reduce SMAD3 ubiquitination, which leads to promoting nuclear translocation of SMAD3 and autoregulation of SMAD3 transcription. Moreover, high PCK2 mRNA expression was significantly associated with poor survival in TNBC patients. In conclusion, our study revealed for the first time that PCK2 activates TGF-beta/SMAD3 signaling by regulating the expression and phosphorylation of SMAD3 by inhibiting TRIM67-mediated SMAD3 ubiquitination and promoting the stimulatory effect of TGF-beta to promote TNBC invasion. The regulatory effect of PCK2 on mTOR and TGF-beta/SMAD3 signaling suggests that PCK2 is a potential therapeutic target for suppressing TNBC progression.
    Date: 2025-03-13
    Relation: Cancer Biology and Therapy. 2025 Mar 13;26(1):Article number 2478670.
    Link to: http://dx.doi.org/10.1080/15384047.2025.2478670
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1538-4047&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:001445508900001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=105000456381
    Appears in Collections:[Hui-Jen Tsai] Periodical Articles
    [Wen-Chun Hung] Periodical Articles
    [Shih-Sheng Jiang] Periodical Articles

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