國家衛生研究院 NHRI:Item 3990099045/1759
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1759


    Title: Primer Design Assistant (PDA): a web-based primer design tool
    Authors: Chen, SH;Lin, CY;Cho, CS;Lo, CZ;Hsiung, CA
    Contributors: Division of Biostatistics and Bioinformatics
    Abstract: Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers. It runs in a Linux-Apache-MySQL-PHP structure on a PC equipped with dual CPU (Intel Pentium III 1.4 GHz) and 512 Mb of RAM. A succinct user interface of PDA is accomplished by built-in parameters setting. Advanced options on 5' GC content, 3' GC content, dimer check and hairpin check are available. The option of covered region constrains the PCR product to cover a user-defined segment. PDA accepts single sequence query or multiple ones in FASTA format. It produces optimal and homogenous primer pairs that meet the need in experimental design with large-scaled PCR amplifications. Considering the system loading, the size of a submitted sequence is limited to 10 kb and the total sequence number in a query is limited to 20. The authors may be contacted regarding other requirements for primer design. The web application can be found at http://dbb.nhri.org.tw/primer/.
    Keywords: Biochemistry & Molecular Biology
    Date: 2003-07-01
    Relation: Nucleic Acids Research. 2003 Jul;31(13):3751-3754.
    Link to: http://dx.doi.org/10.1093/nar/gkg560
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-1048&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000183832900102
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0043122991
    Appears in Collections:[Chao A. Hsiung] Periodical Articles
    [Chung-Yen Lin(1999-2005)] Periodical Articles

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