國家衛生研究院 NHRI:Item 3990099045/1762
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1762


    Title: Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus
    Authors: Juang, JL;Chen, TC;Jiang, SS;Hsiung, CA;Chen, WC;Chen, GW;Lin, SM;Lin, JH;Chiu, SC;Lai, YK
    Contributors: Division of Biostatistics and Bioinformatics;Division of Molecular and Genomic Medicine
    Abstract: An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.
    Keywords: Medicine, Research & Experimental;Pathology
    Date: 2004-09
    Relation: Laboratory Investigation. 2004 Sep;84(9):1085-1091.
    Link to: http://dx.doi.org/10.1038/labinvest.3700136
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0023-6837&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000223429900003
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4344669286
    Appears in Collections:[Chao A. Hsiung] Periodical Articles
    [Jyh-Lyh Juang] Periodical Articles
    [Shih-Sheng Jiang] Periodical Articles

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