國家衛生研究院 NHRI:Item 3990099045/1862
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1862


    Title: Amantadine-resistant influenza A virus in Taiwan
    Authors: Shih, SR;Lee, CN;Tsai, HR;Chen, GW;Tsao, KC
    Contributors: Division of Biostatistics and Bioinformatics
    Abstract: Background and purpose: Amantadine and rimantadine have been used for treatment and prophylaxis of influenza A virus infection. We examined the amantadine susceptibility of field isolates of influenza A virus in Taiwan from 1996 to 1998 to monitor, the presence of resistant strains. Methods: Eighty-four field isolates of influenza A virus were examined for resistance to amantadine by plaque inhibition assay. Virus isolates with amantadine. 50% inhibitory concentrations (IC50) greater than 0.9 mug were chosen for sequence analysis Of the M gene that is the molecular target for amantadine/rimantadine. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the viral RNA. RT-PCR products were examined and purified by agarose gel electrophoresis for further sequence analysis. The Genetics Computer Group Sequence Analysis Package and the neighbor-joining method listed in the Molecular Evolutionary Genetic Analysis package were used for phylogenetic analysis. Results: One field strain was amantadine resistant (IC50 > 10 mug/mL), with a mutation (position 31, serine to asparagine) in the M2 protein. The resistant virus was isolated from a non-immunocompromised child without a history of amantadine/rimantadine treatment. None of the family members reported previous exposure to amantadine/rimantadine. Conclusions: In this series, amantadine-resistant influenza A (H1N1) virus was isolated from a non-immunocompromised Taiwanese child without a known history of exposure to this drug. Resistant field isolates were rare. Due to the increasing use of amantadine/rimantadine in Taiwan, continued surveillance for amantadine/rimantadine-resistant influenza A viruses is warranted.
    Keywords: Medicine, General & Internal
    Date: 2001-09
    Relation: Journal of the Formosan Medical Association. 2001 Sep;100(9):608-612.
    Link to: http://ajws.elsevier.com/ajws3/abstract.asp?art_id=5254&art_journals=12
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0929-6646&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000173016200006
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034748721
    Appears in Collections:[Others] Periodical Articles

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