國家衛生研究院 NHRI:Item 3990099045/1863
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    題名: Serotype-specific detection of enterovirus 71 in clinical specimens by DNA microchip array
    作者: Shih, SR;Wang, YW;Chen, GW;Chang, LY;Lin, TY;Tseng, MC;Chiang, C;Tsao, KC;Huang, CG;Shio, MR;Tai, JH;Wang, SH;Kuo, RL;Liu, WT
    貢獻者: Division of Biostatistics and Bioinformatics
    摘要: Enterovirus 71 is an important pathogen that causes high morbidity and mortality in children in Taiwan. Virus isolation in cell cultures has been the standard method for enterovirus 71 identification in Clinical Virology Laboratories. However, virus isolation takes 5-10 days when using cell culture. A microchip for enterovirus 71 detection was developed as an alternative diagnostic method. The novel approach is based on hybridization of amplified DNA specimens with oligonucleotide DNA probes immobilized on a microchip. Two oligonucletides were used as detection probes, the pan-enterovirus sequence located in the 5'-noncoding region (5'-NCR) and the enterovirus 71-specific sequence located in the VP2 region. The diagnostic procedure takes 6 h. One hundred specimens identified as enteroviruses by viral cultures were tested using this microchip, including 67 enterovirus 71 specimens. The sensitivity of the novel method is 89.6% and its specificity is 90.9%. The enterovirus 71-microchip can detect the amplicon derived from viral RNA corresponding to 1-10 virions in a clinical specimen. Microchip array is a potential diagnostic method for identification of enterovirus in the future. (C) 2003 Elsevier Science B.V. All rights reserved.
    關鍵詞: Biochemical Research Methods;Biotechnology & Applied Microbiology;Virology
    日期: 2003-07
    關聯: Journal of Virological Methods. 2003 Jul;111(1):55-60.
    Link to: http://dx.doi.org/10.1016/S0166-0934(03)00151-4
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0166-0934&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000184079600007
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10744222479
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