國家衛生研究院 NHRI:Item 3990099045/1961

NHRI > National Institute of Infectious Diseases and Vaccinology > Leung-Kei Siu > Periodical Articles > Item 3990099045/1961

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Title:	High prevalence of phagocytic-resistant capsular serotypes of Klebsiella pneumoniae in liver abscess
Authors:	Lin, JC;Chang, FY;Fung, CP;Xu, JZ;Cheng, HP;Wang, JJ;Huang, LY;Siu, LK
Contributors:	Division of Clinical Research
Abstract:	To better understand the role of capsular polysaccharide (CPS) K1 or K2 in Klebsiella pneumoniae liver abscess as well as the development of metastasis to eye, neutrophil phagocytosis of 70 CPS isolates including K1 (n = 23)/K2 (n = 10), non-K1/K2 (n = 37) was evaluated by flow cytometry, fluorescence imaging, and electron microscopy. K1/K2 isolates were significantly more resistant to phagocytosis (P < 0.0001) than non-K1/K2 isolates and displayed increased resistance to intracellular killing. Although mucoid phenotype (M-type) K1/K2 isolates were significantly more resistant to phagocytosis (P = 0.0029) than NI-type non-K1/K2, no significant difference in the phagocytosis rate was observed between K1/K2 isolates with M-type and non-M-type (P = 0.0924). Mucoidy is an associated factor that was predominant in K1/K2 isolates, but which itself is not an independent influence on phagocytic resistance. The K1/K2 CPS proved significantly more resistant to phagocytosis than non-K1/K2 CPS in liver abscess isolates (P < 0.0001) and non-abscess isolates (P = 0.0001), suggesting that K1/K2 isolates were generally more virulent in both liver abscess and in non-liver abscess conditions. These findings indicate that resistance of CPS K1 or K2 K. pneumoniae to phagocytosis and intracellular killing presumably contributes to their high prevalence in liver abscess and uniquely in endophthalmitis. (C) 2004 Elsevier SAS. All rights reserved.
Keywords:	Immunology;Microbiology;Virology
Date:	2004-11
Relation:	Microbes and Infection. 2004 Nov;6(13):1191-1198.
Link to:	http://dx.doi.org/10.1016/j.micinf.2004.06.003
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1286-4579&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000224728700008
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=5644285349
Appears in Collections:	[Leung-Kei Siu] Periodical Articles

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