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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/2118
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| Title: | Establishment of a mini-gene expression database for bladder tumor |
| Other Titles: | 建立膀胱癌細胞表達之小型基因資料庫 |
| Authors: | Li, C;Chen, CC;Yang, TH;Tzai, TS;Huang, CH;Liou, JT;Su, IJ;Tzeng, CC;Chiu, AW;Shieh, B |
| Contributors: | Computer Center |
| Abstract: | Background and Purpose: Transition cell carcinoma has been diagnosed mostly in urinary bladder in Southern Taiwan and has an exceptionally high mortality rate. To identify die genes associated with bladder cancer, we investigated differential gene expression. Six bladder tumor cDNA libraries were constructed and their sequences were compared and analized. Methods: mRNA from bladder tumor cell lines or tissue samples were used to construct two regular cDNA libraries and four subtractive cDNA libraries after subtractive hybridization with cDNA derived from normal bladder epithelial cells. Subsequent, more than 100 cDNA inserts from each library were randomly isolated and sequenced, followed by sequence comparisons with nucleotide and protein sequence databases. Results: After searching the Basic Local Alignment Search Tool (Blast) databases, the cDNA nucleotide sequences were grouped into novel, known, and common gene categories. Since tumor nucleotide sequences are informative and valuable for research, they were organized as a mini-gene expression database (http://bladder. nhri.org.tw/). Interestingly, in one subtractive cDNA library, the ATPase 6 gene was found to be highly expressed in normal bladder epithelial cells and elevated levels of ATPase 6 mRNA were later confirmed by reverse transcription-polymerase chain reaction. However. the role of ATPase 6 in bladder tumorigenesis remains to be investigated. Conclusions: The establishment of this database is an important step to enable systematic screening for bladder tumor-associated genes and may also be useful in developing diagnostic and/or therapeutic applications. |
| Keywords: | Medicine, General & Internal |
| Date: | 2002-02 |
| Relation: | Journal of the Formosan Medical Association. 2002 Feb;101(2):104-109. |
| Link to: | http://www.fma.org.tw/fagmag/pdfiles/101_2/101-2-104.pdf |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0929-6646&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000175156300003 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036307082 |
| Appears in Collections: | [其他] 期刊論文
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| 000175156300003.pdf | | 1387Kb | Adobe PDF | 419 | View/Open |
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