國家衛生研究院 NHRI:Item 3990099045/2128
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    題名: Digital cloning: Identification of human cDNAs homologous to novel kinases through expressed sequence tag database searching
    作者: Chen, HC;Kung, HJ;Robinson, D
    貢獻者: Division of Molecular and Genomic Medicine;Institute of Biotechnology and Pharmaceutical Research
    摘要: Identification of novel kinases based on their sequence conservation within kinase catalytic domain has relied so far on two major approaches, low-stringency hybridization of cDNA libraries, and PCR method using degenerate primers. Both of these approaches at times are technically difficult and time-consuming. We have developed a procedure that can significantly reduce the time and effort involved in searching for novel kinases and increase the sensitivity of the analysis. This procedure exploits the computer analysis of a vast resource of human cDNA sequences represented in the expressed sequence tag (EST) database. Seventeen novel human cDNA clones showing significant homology to serine/threonine kinases, including STE-20, CDK- and YAK-related family kinases, were identified by searching EST database. Further sequence analysis of these novel kinases obtained either directly from EST clones or from PCR-RACE products confirmed their identity as protein kinases. Given the rapid accumulation of the EST database and the advent of powerful computer analysis software, this approach provides a fast, sensitive, and economical way to identify novel kinases as well as other genes from EST database.
    關鍵詞: Medicine, Research & Experimental
    日期: 1998-03
    關聯: Journal of Biomedical Science. 1998 Mar;5(2):86-92.
    Link to: http://dx.doi.org/10.1007/BF02258361
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000074139900004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031747935
    顯示於類別:[陳華鍵(1997-2001)] 期刊論文
    [龔行健] 期刊論文

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