國家衛生研究院 NHRI:Item 3990099045/2209
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    题名: Transcriptional repression by Drosophila methyl-CpG-binding proteins
    作者: Roder, K;Hung, MS;Lee, TL;Lin, TY;Xiao, HY;Isobe, KI;Juang, JL;Shen, CKJ
    贡献者: Division of Molecular and Genomic Medicine
    摘要: C methylation at genomic CpG dinucleotides has been implicated in the regulation of a number of genetic activities during vertebrate cell differentiation and embryo development. The methylated CpG could induce chromatin condensation through the recruitment of histone deacetylase (HDAC)-containing complexes by methyl-CpG-binding proteins. These proteins consist of the methylated-DNA binding domain (MBD). Unexpectedly, however, several studies have identified MBD-containing proteins encoded by genes of Drosophila melanogaster, an invertebrate species supposed to be void of detectable m(5)CpG. We now report the genomic structure of a Drosophila gene, dMBD2/3, that codes for two MBD-containing, alternatively spliced, and developmentally regulated isoforms of proteins, dMBD2/3 and dMBD2/3 Delta. Interestingly, in vitro binding experiments showed that as was the case for vertebrate MBD proteins, dMBD2/3 Delta could preferentially recognize m(5)CpG-containing DNA through its MBD. Furthermore, dMBD2/3 Delta as well as one of its orthologs in mouse, MBD2b, could function in human cells as a transcriptional corepressor or repressor. The activities of HDACs appeared to be dispensable for transcriptional repression by dMBD2/3 Delta. Finally, dMBD2/3 Delta also could repress transcription effectively in transferred Drosophila cells. The surprisingly similar structures and characteristics of the MBD proteins as well as DNA cytosine (C-5) methyltransferase-related proteins in Drosophila and vertebrates suggest interesting scenarios for their roles in eukaryotic cellular functions.
    关键词: Biochemistry & Molecular Biology;Cell Biology
    日期: 2000-10
    關聯: Molecular and Cellular Biology. 2000 Oct;20(19):7401-7409.
    Link to: http://dx.doi.org/10.1128/MCB.20.19.7401-7409.2000
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0270-7306&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000089268700036
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033826338
    显示于类别:[莊志立] 期刊論文

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