國家衛生研究院 NHRI:Item 3990099045/2270
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    题名: Surfection: a new platform for transfected cell arrays
    作者: Chang, FH;Lee, CH;Chen, MT;Kuo, CC;Chiang, YL;Hang, CY;Roffler, S
    贡献者: Division of Molecular and Genomic Medicine
    摘要: Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid-cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface-mediated transfection method, termed 'surfection', can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening.
    关键词: Biochemistry & Molecular Biology
    日期: 2004-02
    關聯: Nucleic Acids Research. 2004 Feb;32(3):e33-e33.
    Link to: http://dx.doi.org/10.1093/nar/gnh029
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-1048&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000220490400050
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=2342593280
    显示于类别:[黃奇英(1998-2005)] 期刊論文

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