國家衛生研究院 NHRI:Item 3990099045/2287

NHRI > Institute of Molecular and Genomic Medicine > Shiou-Hwei Yeh(2001-2005) > Periodical Articles > Item 3990099045/2287

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2287

Title:	Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA
Authors:	Huang, LR;Chiu, CM;Yeh, SH;Huang, WH;Hsueh, PR;Yang, WZ;Yang, JY;Su, IJ;Chang, SC;Chen, PJ
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	Severe acute respiratory syndrome (SARS)-CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS-CoV, we evaluated nucleocapsid (N) and spike (S) proteins-specific antibodies in patients' sera by Western blotting and enzyme-linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS-CoV were purified from transformed E. coli. Serum specimens from 40 SARS-CoV-infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT-PCR-confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein-specific antibodies whereas only 47.5% of the samples were positive for S protein-specific antibodies. Using N protein-based ELISA, 10 out of the 12 patients were positive for N protein-specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS-CoV infection. Antibodies against SARS-CoV N protein appear at the early stage of infection, therefore, N protein-based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS-CoV infection.
Keywords:	Virology
Date:	2004-07
Relation:	Journal of Medical Virology. 2004 Jul;73(3):338-346.
Link to:	http://dx.doi.org/10.1002/jmv.20096
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0146-6615&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000221677700003
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84984533509
Appears in Collections:	[Shiou-Hwei Yeh(2001-2005)] Periodical Articles

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