國家衛生研究院 NHRI:Item 3990099045/2314
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2314


    Title: Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17
    Authors: Chung, WJ;Shu, HY;Lu, CY;Wu, CY;Tseng, YH;Tsai, SF;Lin, CH
    Contributors: Division of Molecular and Genomic Medicine
    Abstract: The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2-DE and the well-resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence-related proteins. We detected 22 up-regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti-host defense protein, Ohr. Thus, further functional studies of these up-regulated proteins with respect to their roles in XCC pathogenicity are suggested.
    Keywords: Biochemical Research Methods;Biochemistry & Molecular Biology
    Date: 2007-06
    Relation: Proteomics. 2007 Jun;7(12):2047-2058.
    Link to: http://dx.doi.org/10.1002/pmic.200600647
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1615-9853&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000247642100010
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34347371488
    Appears in Collections:[Shih-Feng Tsai] Periodical Articles

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