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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/2332
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| Title: | Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43 |
| Authors: | Chen, YT;Chang, HY;Lai, YC;Pan, CC;Tsai, SF;Peng, HL |
| Contributors: | Division of Molecular and Genomic Medicine |
| Abstract: | We have determined the entire DNA sequence of pLVPK, which is a 219-kb virulence plasmid harbored in a bacteremic isolate of Klebsiella pneumoniae. A total of 251 open reading frames (ORFs) were annotated, of which 37% have homologous genes of known function, 31% match the hypothetical genes in the GenBank database, and the remaining 32% are novel sequences. The obvious virulence-associated genes carried by the plasmid are the capsular polysaccharide synthesis regulator rmpA and its homolog rmpA2, and multiple iron-acquisition systems, including iucABCDiutA and iroBCDN siderophore gene clusters, Mesorhizobium loti fepBC ABC-type transporter, and Escherichia coli fecIRA, which encodes a Fur-dependent regulatory system for iron uptake. In addition, several gene clusters homologous with copper, silver, lead, and tellurite resistance genes of other bacteria were also identified. Identification of a replication origin consisting of a repA gene lying in between two sets of iterons suggests that the replication of pLVPK is iteron-controlled and the iterons are the binding sites for the repA to initiate replication and maintain copy number of the plasmid. Genes homologous with E. coli sopA/sopB and parA/parB with nearby direct DNA repeats were also identified indicating the presence of an F plasmid-like partitioning system. Finally, the presence of 13 insertion sequences located mostly at the boundaries of the aforementioned gene clusters suggests that pLVPK was derived from a sequential assembly of various horizontally acquired DNA fragments. |
| Keywords: | Genetics & Heredity |
| Date: | 2004-08-04 |
| Relation: | Gene. 2004 Aug;337:189-198. |
| Link to: | http://dx.doi.org/10.1016/j.gene.2004.05.008 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0378-1119&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000223383000019 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=3242771398 |
| Appears in Collections: | [蔡世峯] 期刊論文
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| 000223383000019.pdf | | 1928Kb | Adobe PDF | 640 | View/Open |
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