國家衛生研究院 NHRI:Item 3990099045/2341
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    題名: Genomic shotgun array: a procedure linking large-scale DNA sequencing with regional transcript mapping
    作者: Li, LH;Li, JC;Lin, YF;Lin, CY;Chen, CY;Tsai, SF
    貢獻者: Division of Molecular and Genomic Medicine;Division of Biostatistics and Bioinformatics
    摘要: To facilitate transcript mapping and to investigate alterations in genomic structure and gene expression in a defined genomic target, we developed a novel microarray-based method to detect transcriptional activity of the human chromosome 4q22-24 region. Loss of heterozygosity of human 4q22-24 is frequently observed in hepatocellular carcinoma (HCC). One hundred and eighteen well-characterized genes have been identified from this region. We took previously sequenced shotgun subclones as templates to amplify overlapping sequences for the genomic segment and constructed a chromosome-region-specific microarray. Using genomic DNA fragments as probes, we detected transcriptional activity from within this region among five different tissues. The hybridization results indicate that there are new transcripts that have not yet been identified by other methods. The existence of new transcripts encoded by genes in this region was confirmed by PCR cloning or cDNA library screening. The procedure reported here allows coupling of shotgun sequencing with transcript mapping and, potentially, detailed analysis of gene expression and chromosomal copy of the genomic sequence for the putative HCC tumor suppressor gene(s) in the 4q candidate region.
    關鍵詞: Biochemistry & Molecular Biology
    日期: 2004-02
    關聯: Nucleic Acids Research. 2004 Feb;32(3):e27-e27.
    Link to: http://dx.doi.org/10.1093/nar/gnh025
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-1048&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000220490400044
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=2342478506
    顯示於類別:[蔡世峯] 期刊論文
    [林仲彥(1999-2005)] 期刊論文

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