國家衛生研究院 NHRI:Item 3990099045/2352

NHRI > Institute of Molecular and Genomic Medicine > King-Song Jeng(1997-2002) > Periodical Articles > Item 3990099045/2352

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2352

Title:	Varied assembly and RNA editing efficiencies between genotypes I and II hepatitis D virus and their implications
Authors:	Hsu, SC;Syu, WJ;Sheen, IJ;Liu, HT;Jeng, KS;Wu, JC
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	The mechanisms that link genotypes of hepatitis D virus (HDV) with clinical outcomes have not yet been elucidated. Genotypic variations are unevenly distributed along the sequences of hepatitis delta antigens (HDAgs). Of these variations, the packaging signal at the C-terminus has a divergence of 74% between genotypes I and II. In this report, we address the issue of whether these high variations between genotypes affect assembly efficiency of HDV particles and editing efficiency of RNA. Viral package systems of transfection with expression plasmids of hepatitis B surface antigen and HDAgs or whole genomes of HDV consistently indicate that the package efficiency of genotype I HDV is higher than that of genotype II. Segment swapping of large-form HDAg indicates that the C-terminal 19-residue region plays a key role for the varied assembly efficiencies. Also, the editing efficiency of genotype I HDV is higher than that of genotype II. The nucleotide and structural changes surrounding the editing site may explain why genotype II HDV has a low RNA editing efficiency. The findings of in vitro assembly systems were further supported by the observations that patients infected with genotype II had significantly lower alanine transaminase (ALT) levels, more favorable outcomes (P <.05), and a trend to have lower serum HDV RNA levels as compared with those infected with genotype I HDV (P =.094). In conclusion, genotype II HDV secretes fewer viral particles than genotype I HDV does, which in turn: may reduce the extent of infection of hepatocytes and result in less severe hepatic inflammation.
Keywords:	Gastroenterology & Hepatology
Date:	2002-03
Relation:	Hepatology. 2002 Mar;35(3):665-672.
Link to:	http://dx.doi.org/10.1053/jhep.2002.31777
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0270-9139&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000174096700022
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036185853
Appears in Collections:	[King-Song Jeng(1997-2002)] Periodical Articles

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