國家衛生研究院 NHRI:Item 3990099045/2358
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    题名: Coat color-tagged green mouse with EGFP expressed from the RNA polymerase II promoter
    作者: Hsiao, YC;Chang, HH;Tsai, CY;Jong, YJ;Horng, LS;Lin, SF;Tsai, TF
    贡献者: Division of Molecular and Genomic Medicine
    摘要: Laborious molecular genotyping and variegated gene expression are two widely encountered issues for transgenic mouse studies. To facilitate genotyping in the FVB/N albino background and to reduce variegated expression, we successfully generated double-tagged transgenic mice for direct visual genotyping with the coat color phenotype derived from tyrosinase cDNA driven by the tyrosinase promoter and with simultaneous high enhanced green fluorescent protein (EGFP) expression driven by the promoter of RNA polymerase II large subunit gene. Incorporation of insulator into a transgene construct achieved high efficiency of transgene expression in more than 90% of the founders. EGFP was detected as early as the one-cell fertilized egg and lasted for the whole embryo development, as well as in all of the adult tissues examined. The coat color-tagged green mice offer opportunities in applications such as tissue transplantation, lineage tracing, chimera biology, RNA interference, and other transgenic studies. (C) 2004 Wiley-Liss, Inc.
    关键词: Developmental Biology;Genetics & Heredity
    日期: 2004-06
    關聯: Genesis. 2004 Jun;39(2):122-129.
    Link to: http://dx.doi.org/10.1002/gene.20038
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1526-954X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000222490500008
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=3142702789
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