國家衛生研究院 NHRI:Item 3990099045/2365
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 916241      線上人數 : 1422
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/2365


    題名: Blockage of apoptotic signaling of transforming growth factor-beta in human hepatoma cells by carboxyfullerene
    作者: Huang, YL;Shen, CKF;Luh, TY;Yang, HC;Hwang, KC;Chou, CK
    貢獻者: Division of Molecular and Genomic Medicine
    摘要: Transforming growth factor-beta (TGF-beta) has been shown to induce apoptosis in normal hepatocytes and hepatoma cells both in vivo and in vitro. However, the mechanism by which TGF-beta induces apoptosis is not clear. The antiapoptotic activity of antioxidants including N-acetyl-L-cysteine (Ac-Cys), ascorbic acid and a novel free radical scavenger, carboxyfullerene (C-60) on TGF-beta-treated human hepatoma Hep3B cells was examined. Only the water-soluble hexacarboxylic acid derivative of C-60 was found to prevent TGF-beta-induced apoptosis. Antiapoptotic activity of C-60 correlated its ability to eliminate TGF-beta-generated reactive oxygen species (ROSs). However, C-60 did not interfere with TGF-beta-activated PAI-1 promoter activity in the Hep3B cells. These results indicate that the signaling pathway of TGF-beta-induced apoptosis may be related to the generation of ROSs and may be uncoupled from the TGF-beta-activated gene promoter activity. Furthermore, the regioisomer of C-60 with a C-3 symmetry was more potent in protecting cells from apoptosis than that with a D-3 symmetry, and the C-3 isomer had stronger interactions with lipid bilayers than the D-3 isomer. The spectroscopic analysis revealed that the C-3 isomer had stranger interactions with artificial lipid bilayers than the D-3 isomer. Therefore, our study indicates that C-60 may interact with membrane to eliminate TGF-beta-induced ROSs and to prevent apoptosis occur in human hepatoma cells.
    關鍵詞: Biochemistry & Molecular Biology
    日期: 1998-05-15
    關聯: European Journal of Biochemistry. 1998 May;254(1):38-43.
    Link to: http://dx.doi.org/10.1046/j.1432-1327.1998.2540038.x
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000073841100006
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032524008
    顯示於類別:[其他] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    000073841100006.pdf313KbAdobe PDF659檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋