國家衛生研究院 NHRI:Item 3990099045/2486

NHRI > National Institute of Environmental Health Sciences > Louis W. Chang(1999-2009) > Periodical Articles > Item 3990099045/2486

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2486

Title:	Low concentrations of arsenic induce vascular endothelial growth factor and nitric oxide release and stimulate angiogenesis in vitro
Authors:	Kao, YH;Yu, CL;Chang, LW;Yu, HS
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	Arsenic (As) is widely distributed in nature, and its contamination in drinking water remains a major public health problem. Exposure to As may lead to degenerative peripheral vascular diseases. The purpose of this study is to clarify the role of As in modulating cell proliferation and in vitro angiogenesis in human umbilical vein endothelial cells (HUVECs) and to scrutinize the contributing factors of these events. The results revealed that lower concentrations (up to 1 muM) of sodium arsenite stimulated HUVEC cell growth. An in vitro angiogenesis assay indicated that low concentrations of As increased vascular tubular formation, which was abrogated by hemoglobin, a potent nitric oxide scavenger. In contrast, higher concentrations of As (> 5 muM) revealed cytotoxicity and inhibition to angiogenesis. We also demonstrated that lower concentrations of As upregulated the expression of constitutive nitric oxide synthase (NOS3) at both transcriptional and translational levels and that lower concentrations of As implicated a modulatory role in vascular endothelial growth factor (VEGF) expression. In addition, low concentrations of As (<1 muM) increased von Willebrand Factor (vWF) antigen expression, whose elevation paralleled the onset of angiogenesis and was considered an early indicator of endothelial activation in tumor metastasis. VEGF and basic fibroblast growth factor can synergistically upregulate the vWF gene expression. Therefore, we conclude that the treatment of HUVECs with As leads to cell proliferation and activation, which preferentially enhances angiogenesis in vitro, possibly via the VEGF-NOS signaling pathway. The molecular mechanism(s) by which As facilitates angiogenesis remains to be elucidated.
Keywords:	Chemistry, Medicinal;Chemistry, Multidisciplinary;Toxicology
Date:	2003-04
Relation:	Chemical Research in Toxicology. 2003 Apr;16(4):460-468.
Link to:	http://dx.doi.org/10.1021/tx025652a
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0893-228X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000182586900004
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037397116
Appears in Collections:	[Louis W. Chang(1999-2009)] Periodical Articles

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