國家衛生研究院 NHRI:Item 3990099045/2681
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    題名: Utilization of Drosophila eye to probe the functions of two mammalian serine/threonine kinases, Snk and HsHPK
    作者: Liu, MA;Huang, AM;Chou, CK;Liaw, GJ;Wu, CW
    貢獻者: National Institute of Cancer Research
    摘要: Here we report a quick functional analysis of two mammalian serine/threonine kinases, a serum inducible kinase (Snk) and Home sapiens hepatoma protein kinase (HsHPK), using Drosophila eye as a model system. We generated transgenic fly lines carrying constructs of both kinases under control of the GAL upstream activating sequence (UAS), Each UAS line was then crossed to a line in which GAL4 expression was driven by one of the following promoters, eyeless (ey), glass or decapentaplegic, Thus, different kinase mutants can be ectopically expressed in a promoter-dependent manner. We observed that the ectopic expression of either the wild-type or active form of Snk driven by the glass promoter resulted in a rough-eye phenotype. Nevertheless, the ectopic expression of HsHPK under the control of the ey promoter resulted in a small-eye phenotype. The results of this study demonstrated that ectopic expression of these two mammalian genes could be achieved by the regulation of Drosophila promoters. In addition, the effects of these ectopically expressed genes on eye development could be an implication of their functions with respect to cell proliferation and differentiation. Thus, Drosophila eye, with the powerful genetic tools and vast information on eye development available, can be a useful system to probe the functions of mam malian genes in the postgenome era.
    關鍵詞: Medicine, Research & Experimental
    日期: 2001-05
    關聯: Journal of Biomedical Science. 2001 May;8(3):270-277.
    Link to: http://dx.doi.org/10.1007/BF02256601
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000168948700006
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85017225186
    顯示於類別:[吳成文(1996-2008)] 期刊論文

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