國家衛生研究院 NHRI:Item 3990099045/2688
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 916018      在线人数 : 1354
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/2688


    题名: Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection
    作者: Chen, JJW;Wu, R;Yang, PC;Huang, JY;Sher, YP;Han, MH;Kao, WC;Lee, PJ;Chiu, TF;Chang, F;Chu, YW;Wu, CW;Peck, K
    贡献者: National Institute of Cancer Research
    摘要: A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane is described and characterized. To quantify the expression of genes and to isolate differentially expressed genes, the southern hybridization process on filter membranes was employed. The levels of gene expression were represented by color intensities generated by colorimetric reactions in place of hazardous radioisotopes or costly laser-induced fluorescence detection. The gene expression patterns on nylon membranes were digitized by devices such as an economical flatbed scanner or a digital camera. The quantitative information of gene expression was retrieved by image analysis software. Quantitative comparison of the northern dot-blotting method with the microarray system is described. Applications employing single-color detection as well as dual-color detection to isolate differentially expressed genes among thousands of genes are demonstrated. (C) 1998 Academic Press
    关键词: Biotechnology & Applied Microbiology;Genetics & Heredity
    日期: 1998-08-01
    關聯: Genomics. 1998 Aug;51(3):313-324.
    Link to: http://dx.doi.org/10.1006/geno.1998.5354
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0888-7543&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000075674000001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032143452
    显示于类别:[吳成文(1996-2008)] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    000075674000001.pdf8810KbAdobe PDF488检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈