國家衛生研究院 NHRI:Item 3990099045/2796
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2796


    Title: Differentiation of pheochromocytoma PC12 cells induced by human urine extract and the involvement of the extracellular signal-regulated kinase signaling pathway
    Authors: Yao, CJ;Lai, GM;Chan, CF;Yang, YY;Liu, FC;Chuang, SE
    Contributors: National Institute of Cancer Research
    Abstract: Objective: The aim of this study was to investigate the effects of a human urine preparation on the differentiation of tumor cells. Design: The pheochromocytoma PC12 cells were used to examine the effects of a human urine preparation, CDA-2 on the induction of differentiation markers, neurofilaments, and compared with that induced by nerve growth factor (NGF). The MAPK/ERK kinase (MEK) inhibitor U0126 was used to examine the involvement of mitogen-activatel protein kinase (MAPK) signaling pathway in this differentiation inducing effect. Results: We find that CDA-2 could induce differentiation of pheochromocytoma PC12 cells, as evidenced by the markedly increased expression of neurofilaments to a level comparable to those induced by NGF. This phenomenon was accompanied by the phosphorylation of extracellular-signal-regulated kinase (ERK) and could be inhibited by the MEK inhibitor, U0126. Conclusions: Our results demonstrate the presence of active components in the human urine extract that can induce differentiation of PC12 cells and may involve the ERK signaling pathway. This may provide new in-sights for seeking novel differentiation agents and offer hope for cancer patients.
    Keywords: Integrative & Complementary Medicine
    Date: 2005-10
    Relation: Journal of Alternative and Complementary Medicine. 2005 Oct;11(5):903-908.
    Link to: http://dx.doi.org/10.1089/acm.2005.11.903
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1075-5535&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000233615900031
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=28444443722
    Appears in Collections:[Shuang-En Chuang] Periodical Articles
    [Gi-Ming Lai(2004-2008)] Periodical Articles

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