國家衛生研究院 NHRI:Item 3990099045/2854
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    题名: Characterization of monoclonal antibody to the Epstein-Barr virus BHRF1 protein, a homologue of Bcl-2
    作者: Chou, SP;Tsai, CH;Li, LY;Liu, MY;Chen, JY
    贡献者: National Institute of Cancer Research
    摘要: A monoclonal antibody (MAb), designated 3E8, was produced against the Epstein-Barr virus BHRF1 which is a viral homologue of the anti-apoptotic protein Bcl-2. The MAb recognized the BHRF1 protein in extracts from EBV-containing cell lines after activation and EBV-negative cell lines transfected by the BHRF1 gene. Epitope mapping by Western blot analysis revealed that the antibody bound region encompassing amino acid residues 28 - 33 of the BHRF1. In addition to immunoblotting, the MAb could be applied widely in detection of the BHRF1 in many assays, including immunofluorescence assay, immunohistochemistry, enzyme-linked immunosorbent assay and immunoprecipitation. Most of all, when used in immunoprecipitation experiments, the MAb 3E8 showed a better effect than the existing anti-BHRF1 MAbs since radioactive isotopes were not required to intensify signals of its target antigen. Based on its great use in a variety of immunological reactions, it is a powerful tool to elucidate the biological functions of BHRF1.
    关键词: Biochemical Research Methods;Biotechnology & Applied Microbiology;Immunology
    日期: 2004-02
    關聯: Hybridoma and Hybridomics. 2004 Feb;23(1):29-37.
    Link to: http://dx.doi.org/10.1089/153685904322772006
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000188971300005
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=1242339703
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