國家衛生研究院 NHRI:Item 3990099045/2858
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2858


    Title: Immune responses to Epstein-Barr virus lytic proteins in patients with nasopharyngeal carcinoma
    Authors: Liu, MY;Huang, YT;Sheen, TS;Chen, JY;Tsai, CH
    Contributors: National Institute of Cancer Research
    Abstract: Immune responses to three Epstein-Barr virus (EBV) lytic proteins, DNase, thymidine kinase (TK), and BMRF-1 gene products (50/52 kDa diffused early antigen, EA-D complex) were determined in EBV-infected control individuals and patients with nasopharyngeal carcinoma (NPC). Immunofluorescence assays (IFA) Were used to detect their humoral immune responses using recombinant EBV lytic proteins expressed in a baculovirus system as antigens. Cell proliferation assays were performed to evaluate their cellular immune responses by monitoring H-3-thymidine incorporation. Seventy patients with NPC and 32 non-cancer controls were analyzed. The results of IFA showed antibody titers to all three EBV lytic proteins to be higher in the patients with NPC especially for the IgA class. Positivity rates of the three IgA antibodies also were higher in the patients with NPC population. Furthermore, the profiles of the IgA antibodies correlated with those to total early antigens (EA) expressed in the early phase and viral capsid antigen (VCA) expressed in the late phase, of EBV replication. The most interesting finding was that antibody titers to the three EBV lytic proteins were associated significantly with metastases of cervical lymph nodes in patients with NPC. As for cellular immunity to the EA-D complex and DNase, weak responses were observed in the cell proliferation assays. Peripheral blood cells from most individuals could not be stimulated to proliferate, except for a few patients with NPC whose antibody titers against the EA-D complex and DNase also were very high. (C) 2004 Wiley-Liss, Inc.
    Keywords: Virology
    Date: 2004-08
    Relation: Journal of Medical Virology. 2004 Aug;73(4):574-582.
    Link to: http://dx.doi.org/10.1002/jmv.20128
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0146-6615&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000222317200012
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=3042821644
    Appears in Collections:[Jen-Yang Chen] Periodical Articles

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