國家衛生研究院 NHRI:Item 3990099045/2865
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2865


    Title: Differential regulation of Streptococcus mutans gtfBCD genes in response to copper ions
    Authors: Chen, PM;Chen, JY;Chia, JS
    Contributors: National Institute of Cancer Research
    Abstract: To persist in the oral cavity, bacteria must be able to tolerate environmental fluctuation, particularly in pH, nutrients, and essential elements. Glucosyltransferases B, C, and D of Streptococcus mutans synthesize glucans, and play essential roles in the sucrose-dependent adhesion of the organism to tooth surfaces. Transcriptions of gtfB, gtfC, and gtfD could be differentially regulated through independent promoters. To test the hypothesis that environmental factors frequently encountered in the dental plaque might serve as effector molecules involved in regulation, transcripts of individual gtfs were identified by reverse transcriptase-polymerase chain reaction assay and confirmed by Northern blot analysis using anti-sense RNA probes. When S. mutans was grown in different medium at low pH, differential regulation of the gtfs was observed. More specifically, the transcription and translational expression of gtfD but not gtfB and gtfC was specifically induced by copper ion (Cu2+). The up-regulation was independent of the Cu2+-transport operon copYAZ. These findings support the involvement of Cu2+ as an effector molecule in the regulation of S. mutans gtfD. Nutrient change dominates influence of pH but not the effect of Cu2+.
    Keywords: Microbiology
    Date: 2006-03
    Relation: Archives of Microbiology. 2006 Mar;185(2):127-135.
    Link to: http://dx.doi.org/10.1007/s00203-005-0076-2
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0302-8933&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000235245800006
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=32344443020
    Appears in Collections:[Jen-Yang Chen] Periodical Articles

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