國家衛生研究院 NHRI:Item 3990099045/2904
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 856688      線上人數 : 824
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/2904


    題名: Involvement of Fas (CD95/APO-1) and Fas ligand in apoptosis induced by ganciclovir treatment of tumor cells transduced with herpes simplex virus thymidine kinase
    作者: Wei, SJ;Chao, Y;Shih, YL;Yang, DM;Hung, YM;Yang, WK
    貢獻者: National Institute of Cancer Research
    摘要: Transduction of cancer cells with herpes simplex virus thymidine kinase gene (HSVtk) followed by prodrug ganciclovir (GCV) treatment has been shown to induce apoptosis. In this study, four murine tumors including B16F10 melanoma, NG4TL4 sarcoma, H6 hepatoma and 1MEA 7R.1 hepatoma were found to vary in sensitivity to this gene therapy strategy in vitro but, at effective doses of GCV, the HSVtk-transduced cells of all four tumors showed similar kinetics of early rise in p53 protein levels, then cell cycle S-/G2-phase arrest and finally signs of apoptosis. Immunoblot analyses revealed that Pas (CD95/APO-1), Pas ligand (FasL) and two downstream mediators, RIP and caspase-3 (CPP32, YAMA, Apopain) were increased in GCV-treated HSVtk-transduced tumor cells after the cell cycle arrest and before apoptosis. Increased expression of Fast could also be observed in vivo in HSVtk-transduced tumors induced to regress by GCV treatment. Enzyme measurements using specific substrate showed that the caspase-3 activation followed kinetically the Fast expression. More than half of the HSVtk/GCV-induced cell death could be abrogated by addition to the cell culture medium of a specific antisense oligonucleotide to block Fast synthesis, a recombinant Pas/Fc chimeric protein to compete with Pas receptor for Fast binding, or cell-permeable specific tetrapeptide inhibitors of caspase-3 or caspase-8.
    關鍵詞: Biochemistry & Molecular Biology;Biotechnology & Applied Microbiology;Genetics & Heredity;Medicine, Research & Experimental
    日期: 1999-03
    關聯: Gene Therapy. 1999 Mar;6(3):420-431.
    Link to: http://dx.doi.org/10.1038/sj.gt.3300817
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0969-7128&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000079002400015
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033067561
    顯示於類別:[楊文光(1996-1999)] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    000079002400015.pdf581KbAdobe PDF925檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋