國家衛生研究院 NHRI:Item 3990099045/2911
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2911


    Title: Distinct p53-mediated G(1)/S checkpoint responses in two NIH3T3 subclone cells following treatment with DNA-damaging agents
    Authors: Huang, TS;Kuo, ML;Shew, JY;Chou, YW;Yang, WK
    Contributors: National Institute of Cancer Research
    Abstract: N-3T3 and P-3T3 cells, originally isolated from a NIH3T3 cell clone on the basis of their negative and positive transformation by v-Abl, v-Src and Bcr-Abl, were previously found to show distinct cyclin activity changes following 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment, which is anti-mitogenic for N-3T3 cells and mitogenic for P-3T3 cells. We have found in this study that, while the G(1)/S arrest and cell death induced by serum starvation and TPA treatment in N-3T3 cells did not involve p53-mediated checkpoint or apoptosis, N-3T3 and P-3T3 cells evidently responded differently in these aspects of cell cycle regulation to DNA-damaging agents, methylmethane sulfonate (MMS) and gamma-radiation. In N-3T3 cells, DNA damages elicit cell growth arrest at G(1)/S transition with concomitant accumulation of p53 and p53-inducible Waf1/Cip1 proteins and also signs of apoptosis such as DNA ladder patterns and apoptotic (subgenomic) peak in flow cytograph. Conversely, P-3T3 cells treated with the DNA-damaging agents showed no cell cycle interruption nor accumulation of p53 or Waf1/Cip1. However, both P-3T3 and N-3T3 cells showed the same p53 protein half-life of 40 min or less, the same wild-type p53 DNA sequence and the same co-immunoprecipitable cellular proteins in complexes with p53, suggesting that an alteration in a signal transduction pathway upstream of p53 might account for the evasion of p53-mediated G(1) checkpoint in P-3T3 cells.
    Keywords: Biochemistry & Molecular Biology;Oncology;Cell Biology;Genetics & Heredity
    Date: 1996-08-01
    Relation: Oncogene. 1996 Aug;13(3):625-632.
    Link to: http://www.ncbi.nlm.nih.gov/sites/entrez?myncbishare=nhrilib&Db=pubmed&Cmd=DetailsSearch&Term=8760304
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0950-9232&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:A1996VB32800020
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029814201
    Appears in Collections:[Wen-Kuang Yang(1996-1999)] Periodical Articles
    [Tze-Sing Huang] Periodical Articles

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