國家衛生研究院 NHRI:Item 3990099045/2992

NHRI > National Institute of Cancer Research > Jacqueline Whang-Peng(1996-2007) > Periodical Articles > Item 3990099045/2992

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2992

Title:	Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines
Authors:	Lin, JS;Lu, CW;Huang, CJ;Wu, PF;Robinson, D;Kung, HJ;Chi, CW;Wu, CW;Yang, WK;Whang-Peng, JJK;Lin, WC
Contributors:	National Institute of Cancer Research
Abstract:	Protein kinases play key roles in cellular functions. They are involved in many cellular functions including; signal transduction, cell cycle regulation, cell division, and cell differentiation. Alterations of protein kinase by gene amplification, mutation or viral factors often induce tumor formation and tumor progression toward malignancy. The identification and cloning of kinase genes can provide a better understanding of the mechanisms of tumorigenesis as well as diagnostic tools for tumor staging. In this study, we have used degenerated polymerase-chain-reaction primers according to the consensus catalytic domain motifs to amplify protein kinase genes (protein-tyrosine kinase, PTK, and protein-serine/threonine kinase, PSK) from human stomach cancer cells. Following amplification, the protein kinase molecules expressed in the gastric cancer cells were cloned into plasmid vectors for cloning and sequencing. Sequence analysis of polymerase-chain-reaction. products resulted in the identification of 25 protein kinases, including two novel ones. Expression of several relevant PTK/PSK genes in gastric cancer cells and tissues was further substantiated by RT-PCR using gene-specific primers. The identification of protein kinases expressed or activated in the gastric cancer cells provide the framework to understand the oncogenic process of stomach cancer.
Keywords:	Medicine, Research & Experimental
Date:	1998-03
Relation:	Journal of Biomedical Science. 1998 Mar;5(2):101-110.
Link to:	http://dx.doi.org/10.1007/BF02258363
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000074139900006
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=7144263746
Appears in Collections:	[Jacqueline Whang-Peng(1996-2007)] Periodical Articles

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