國家衛生研究院 NHRI:Item 3990099045/3026

NHRI > National Institute of Cancer Research > Jacqueline Whang-Peng(1996-2007) > Periodical Articles > Item 3990099045/3026

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3026

Title:	Protein tyrosine phosphatase activities are involved in apoptotic cancer cell death induced by GL331, a new homolog of etoposide
Authors:	Huang, TS;Shu, CH;Shih, YL;Huang, HC;Su, YC;Chao, Y;Yang, WK;WhangPeng, J
Contributors:	National Institute of Cancer Research
Abstract:	GL331 is a semisynthetic topoisomerase II inhibitor derived from a plant toxin podophyllotoxin. In 72-h exposure assays, LD(50) values of GL331 range from 0.5 to 2 mu M, which are three- to ten-fold lower than those of its homologous compound etoposide (VP-16), depending on different cancer cell lines including nasopharyngeal, hepatocellular, gastric, cervical and colon cancer types. Apoptotic DNA ladders could be detected when cancer cells were treated with GL331 for 24 h even if the Bcl-2 and Bar protein levels were not altered during the period. Besides acting as topoisomerase II inhibitors, both GL331 and VP-16 decrease the cellular protein tyrosine kinase (PTK) activities in cancer cells. The activities of protein tyrosine phosphatase (PTP) are significantly increased after GL331 treatment but are not affected by VP-16. GL331-induced internucleosomal cleavage can be efficiently prevented by two inhibitors of PTP, sodium orthovanadate and zinc chloride, but not by okadaic acid, which inhibits serine/threonine phosphatase activity. These results indicate that GL331 may induce apoptotic cell death, and that activation of protein tyrosine phosphatases may be involved in this process.
Keywords:	Oncology
Date:	1996-12-20
Relation:	Cancer Letters. 1996 Dec;110(1-2):77-85.
Link to:	http://dx.doi.org/10.1016/S0304-3835(96)04464-3
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0304-3835&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:A1996WD79600011
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0030596182
Appears in Collections:	[Jacqueline Whang-Peng(1996-2007)] Periodical Articles [Wen-Kuang Yang(1996-1999)] Periodical Articles [Tze-Sing Huang] Periodical Articles

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