國家衛生研究院 NHRI:Item 3990099045/3064
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    题名: SFRP1 suppressed hepatoma cells growth through Wnt canonical signaling pathway
    作者: Shih, YL;Hsieh, CB;Lai, HC;Yan, MD;Hsieh, TY;Chao, YC;Lin, YW
    贡献者: National Institute of Cancer Research
    摘要: Oncogenic activation of the Wnt/beta-catenin signaling pathway is common in hepatocellular carcinoma (HCC). The secreted frizzled-related proteins (SFRPs) function as negative regulators of Writ signaling and have important implications for carcinogenesis. Promoter hypermethylation of SFRP genes is common in human cancers. However, the role of SFRPs in HCC is not clear. Recently, we have shown that SFRP1 is frequently downregulated through promoter hypermethylation. To confirm and extend these findings, the methylation status of the other SFRP members, including SFRP2, SFRP4 and SFRP5, was examined by methylation-specific polymerase chain reaction (MS-PCR). Hypermethylation of SFRP genes, except for SFRP4, is frequent in HCCs and the levels found here were significantly higher than those seen in cirrhotic livers, chronic hepatitis livers and normal controls (P < 0. 0001 for SFRP1 and SFRP2, p < 0.05 for SFRP5). To investigate the role of SFRP1 in HCCs, we used re-expression of SFRP1 in Pcatenin-dependent HCC cell lines: Huh6 and HepG2. Restoration of SFRP1 attenuated Writ signaling in those Huh6 hepatoma cells with a beta-catenin gene point mutation, decreased abnormal accumulation of beta-catenin in the nucleus and suppressed cell growth. Conversely, restoration of SFRP1 in HepG2 hepatoma cells with truncated beta catenin could not block the Writ signaling pathway. Furthermore, knocking down SFRP1 by RNA interference in beta-catenin-deficient cell lines (SK-Hep1) stimulated Wnt signaling and promoted cell growth. Our data suggested that SFRP1 suppressed liver cancer cells growth through Wnt canonical signaling. Moreover, beta-cateninindependent noncanonical pathway might be involved in Wnt signaling activation through unknown molecules in HCC.
    关键词: Oncology
    日期: 2007-09-01
    關聯: International Journal of Cancer. 2007 Sep;121(5):1028-1035.
    Link to: http://dx.doi.org/10.1002/ijc.22750
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0020-7136&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000248242500013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34547093863
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