Investigation of the leaves extract of Litsea lii var. nunkao-tahangensis led to the isolation of five new butanolides, litsealiicolide A (1), isolitsealiicolide A (2), litsealiicolide B (3), isolitsealiicolide B (4), and isolitsealiicolide C (5), along with 17 known compounds. Their structures were determined through in-depth spectroscopic and mass-spectrometric analyses. Among the isolates, compounds 1 and 2 were cytotoxic against MCF-7, NCI-H460, and SF-268 cell lines in vitro. Compound 5 and isolinderanolide B (6) showed marginal cytotoxic activity against these three cell lines in vitro.
