國家衛生研究院 NHRI:Item 3990099045/3104

NHRI > National Institute of Infectious Diseases and Vaccinology > Yao Chang > Periodical Articles > Item 3990099045/3104

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Title:	Amplicons on chromosome 3 contain oncogenes induced by recurrent exposure to 12-O-tetradecanoylphorbol-13-acetate and sodium n-butyrate and Epstein-Barr virus reactivation in a nasopharyngeal carcinoma cell line
Authors:	Lee, CH;Fang, CY;Sheu, JJC;Chang, Y;Takada, K;Chen, JY
Contributors:	Division of Clinical Research;National Institute of Cancer Research
Abstract:	Nasopharyngeal carcinoma (NPC) is closely associated with Epstein-Barr virus (EBV) infection and exposure to environmental carcinogens. In this study, an inducible Epstein-Barr virus (EBV) reactivation NPC cell line, NA, was used to investigate the impact of recurrent 12-O-tetradecanoylphorbol-13-acetate-sodium n-butyrate (TPA/SB) treatment and EBV reactivation on chromosomal abnormalities utilizing array-based comparative genomic hybridization (CGH). It was observed that most copy-number aberrations (CNA) were progressively nonrandomly clustered on chromosomes 3, 8, and 9, as the frequency of TPA/SB treatment and EBV reactivation increased. All of the prominent amplicons detected (including 3p14.1, 3p13, 3p12.3, 3p12.2, 3q26.2, 3q26.31, and 3q26.32) were located on chromosome 3, with multiple oncogenes assigned to these sites. The amplification patterns of 3p 12.3 and 3q26.2 were validated using fluorescence in situ hybridization (FISH) analysis. Subsequent quantitative real-time polymerase chain reaction detected increasing expression of ROBO1 and SKIL oncogenes in NA cells harboring higher frequency of TPA/SB treatment and EBV reactivation, consistent with copy-number amplification of these loci. These findings demonstrate that a high incidence of TPA/SB induced-EBV reactivation has a profound influence on the carcinogenesis of NPC through altered DNA copy number.
Keywords:	Oncology;Genetics & Heredity
Date:	2008-08
Relation:	Cancer Genetics and Cytogenetics. 2008 Aug;185(1):1-10.
Link to:	http://dx.doi.org/10.1016/j.cancergencyto.2008.03.014
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000258420900001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=47749124917
Appears in Collections:	[Yao Chang] Periodical Articles [Jen-Yang Chen] Periodical Articles [Chia-Huei Lee] Periodical Articles

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