國家衛生研究院 NHRI:Item 3990099045/3127
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    题名: 17-beta estradiol and hydroxyestradiols interact via the NF-Kappa B pathway to elevate cyclooxygenase 2 expression and prostaglandin E2 secretion in human bronchial epithelial cells
    作者: Ho, CC;Ling, YC;Chang, LW;Tsai, HT;Tsai, MH;Lin, P
    贡献者: Division of Environmental Health and Occupational Medicine
    摘要: Some epidemiological studies suggest women may be at greater risk for lung cancer than men. Hydroxyestradiols (OHE2) are genotoxic and considered as carcinogenic metabolites of estrogens. In this study, we demonstrate that treatment with 0.1 or 1nM 2/4OHE(2) significantly increased intracellular oxidative stress, nuclear factor kappa B (NF-kappa B) activity, and cyclooxygenase-2 (COX-2) expression within 24 h in human bronchial epithelial cells BEAS-2B. Cotreatment with the NF-kappa B inhibitor, Bay 117085, prevented OHE2-induced COX-2 mRNA accumulation, suggesting that OHE2 induced COX-2 expression via the NF-kappa B dependent pathway. Furthermore, cotreatment with 10nM 17-beta estradiol (E-2) significantly enhanced OHE2-increased intracellular oxidative stress and significantly increased not only NF-kappa B activity but also COX-2 levels. As COX-2 participates in biosynthesis of prostaglandin E2 (PGE2), PGE2 secretion was enhanced by the cotreatment of 1nM OHE2 and 10nM E-2. To understand the enhancement mechanism between OHE2 and E-2, cells were cotreated with an antioxidant, N-acetylcysteine (NAC), or NF-kappa B inhibitor, Bay 117085. Both NAC and Bay 117085 prevented the enhancement in COX-2 expression and PGE2 secretion by the cotreatment of E-2 and OHE2 in BEAS-2B cells. Similarly, Bay 117085 prevented PGE2 secretion induced by the cotreatment of E-2 and OHE2 in rat lung slice cultures. These results suggest that E-2 enhanced OHE2-increased intracellular oxidative stress which increased NF-kappa B activity, COX-2 expression, and PGE2 secretion. Elevated COX-2 expression and PGE2 secretion has been shown to increase the risk of cancer development. Our present data suggest a pathway that contributes an epigenetic mechanism to the overall mechanism of carcinogenesis.
    关键词: Toxicology
    日期: 2008-08
    關聯: Toxicological Sciences. 2008 Aug;104(2):294-302.
    Link to: http://dx.doi.org/10.1093/toxsci/kfn096
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1096-6080&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000257789500006
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=47849091814
    显示于类别:[林嬪嬪] 期刊論文
    [張惠華(1999-2009)] 期刊論文

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