國家衛生研究院 NHRI:Item 3990099045/3164

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3164

Title:	Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells
Authors:	Liu, HM;Wu, SH;Lu, CW;Yao, M;Hsiao, JK;Hung, Y;Lin, YS;Mou, CY;Yang, CS;Huang, DM;Chen, YC
Contributors:	Center for Nanomedicine Research
Abstract:	Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPIO@SiO2) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO2-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO2.
Keywords:	Chemistry, Multidisciplinary;Nanoscience & Nanotechnology;Materials Science, Multidisciplinary;Physics, Applied;Physics, Condensed Matter
Date:	2008-05
Relation:	Small. 2008 May;4(5):619-626.
Link to:	http://dx.doi.org/10.1002/smll.200700493
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1613-6810&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000256366100018
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=44949136246
Appears in Collections:	[Dong-Ming Huang] Periodical Articles [Chung-Shi Yang] Periodical Articles

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