國家衛生研究院 NHRI:Item 3990099045/3359

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Title:	Increased aquaglyceroporin 9 expression disrupts arsenic resistance in human lung cancer cells
Authors:	Miao, ZF;Chang, EE;Tsai, FY;Yeh, SC;Wu, CF;Wu, KY;Wang, CJ;Tsou, TC
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	Resistance to chemotherapy is one of the major problems in treatment responses of lung cancer. This study explored the mechanism underlying the arsenic resistance of lung cancer. Four lung cancer cells with different proliferation activity were characterized for cytotoxicity, arsenic influx/efflux, and arsenic effects on intracellular glutathione and 8-hydroxy-2??deoxyguanosine (8-OHdG) production. Our data revealed that relative proliferation potency of these cells was H1299 > A549 > CL3 > H1355. Moreover, A549, H1299, and H1355 were markedly resistant to As2O3 with IC50 ??100 弮M, whereas CL3 was sensitive to As2O3 with IC50 ??11.8 弮M. After treatment with the respective As2O3 at IC50, arsenic influx/efflux activity in CL3 was comparable to those in the other three arsenic-resistant cells. However, differences in glutathione levels and 8-OHdG production were also detected either before or after arsenic treatment, indicating that a certain degree of variation in anti-oxidative systems and/or 8-OHdG repair activity existed in these cell lines. By transfection of an aquaglyceroporin 9 (AQP9) gene, we showed that increased AQP9 expression significantly enhanced arsenic uptake and disrupted arsenic resistance of A549. The present study strongly suggests that membrane transporters responsible for arsenic uptake, such as AQP9, may play a critical role in development of arsenic resistance in human lung cancer cells. 2008 Elsevier Ltd. All rights reserved.
Date:	2009-03
Relation:	Toxicology in Vitro. 2009 Mar;23(2):209-216.
Link to:	http://dx.doi.org/10.1016/j.tiv.2008.11.011
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0887-2333&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000264044000002
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=59249084681
Appears in Collections:	[Tsui-Chun Tsou] Periodical Articles [Kuen-Yuh Wu(2001-2008)] Periodical Articles

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