國家衛生研究院 NHRI:Item 3990099045/3450

NHRI > National Institute of Infectious Diseases and Vaccinology > Ih-Jen Su(2002-2015) > Periodical Articles > Item 3990099045/3450

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Title:	Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression
Authors:	Chang, WW;Su, IJ;Lai, MD;Chang, WT;Huang, W;Lei, HY
Contributors:	Division of Clinical Research
Abstract:	Aim: Toll-like receptor 4 (TLR4) has been shown to be important for bacterial infection, especially to lipopolysaccharide signaling. Its possible role in HBV infection is studied in the present study. Materials and methods: pHBV3.6 plasmid, containing full-length HBV genome was used in the murine model of acute HBV expression by hydrodynamics in vivo transfection. TLR4 normal or mutant mouse strain was compared to investigate the possible role of TLR4 in acute HBV expression. Results: After pHBV3.6 injection, the infiltrating leukocytes expressed TLR4 were observed nearby the HBsAg-expressing hepatocytes. The HBV antigenemia as well as the replication and transcription were higher in TLR4-mutant C3H/HeJ mice than in normal C3H/HeN mice. The HBV-specific immune responses were impaired in the liver or spleen of the C3H/HeJ mice. Their inducible nitric oxide synthase (iNOS) expression on the hepatic infiltrating cells was also impaired. When adoptively transferring splenocytes from C3H/HeN mice to C3H/HeJ mice, the HBV replication was inhibited to the level as that of C3H/HeN. Conclusion: These results suggest that TLR4 plays an anti-HBV role in vivo through the induction of iNOS expression and HBV-specific immune responses after HBV expression. ? 2005 The WJG Press and Elsevier Inc. All rights reserved.
Date:	2005-11
Relation:	World Journal of Gastroenterology. 2005 Nov;11(42):6631-6637.
Link to:	http://www.wjgnet.com/1007-9327/abstract_en.asp?f=6631&v=11
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1007-9327&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000208224700011
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=30444436486
Appears in Collections:	[Ih-Jen Su(2002-2015)] Periodical Articles

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