國家衛生研究院 NHRI:Item 3990099045/3636
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    題名: ALA-PDT results in phenotypic changes and decreased cellular invasion in surviving cancer cells
    作者: Tsai, T;Ji, HT;Chiang, PC;Chou, RH;Chang, WSW;Chen, CT
    貢獻者: National Institute of Cancer Research
    摘要: Background and Objectives: The mechanisms of photo-dynamic therapy (PDT) have been studied on the cellular and tissue levels. However, the cellular behaviors of cancer cells survived from PDT are still not clear. This study attempted to investigate the influence of 5-aminolevulinic acid (ALA)-based PDT on the invasion ability as well as molecular changes in surviving cancer cells and their progeny. Materials and Methods: The systematic effects of ALA-PDT were evaluated using human invasive carcinoma cells (lung adenocarcinoma CL1-5 cells, melanoma A375 cells and breast carcinoma MDA-MB-231 cells). To study the cellular behaviors of surviving cancer cells, PDT-derived variants were established as stable cell lines after consecutive treatment with ALA-PDT. Scratch wound assay and invasion assay were performed to evaluate the migration and invasion ability in the surviving cancer cells and the established PDT-derived variants. RT-PCR and immuno-blot analysis were performed to examine the expression levels of epidermal growth factor receptor (EGFR). Results: Though ALA-PDT caused differential phototox-icity among these invasive carcinoma cells, reduced migration was found in all the surviving cancer cells Compared to parental cancer cells, the established PDT-derived variants exerted significant phenotypic changes of cellular morphology, reduced mitochondrial function and a suppressed cellular invasiveness. Furthermore, correlated with the reduced invasion ability, expression of EGFR was down-regulated in these established PDT-derived variants. Conclusions: Except for direct cell killing, ALA-PDT could reduce EGFR expression and invasion ability of the surviving cancer cells and these effects could further pass to the progeny. The results from this study provide insights into a new mechanism by which PDT might affect cellular behaviors and tumor metastasis.
    日期: 2009-04
    關聯: Lasers in Surgery and Medicine. 2009 Apr;41(4):305-315.
    Link to: http://dx.doi.org/10.1002/lsm.20761
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000265404000009
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=65549100654
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