國家衛生研究院 NHRI:Item 3990099045/4241
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    题名: Mercury resistance and accumulation in Escherichia coli with cell surface expression of fish metallothionein
    作者: Lin, KH;Chien, MF;Hsieh, JL;Huang, CC
    贡献者: Division of Molecular and Genomic Medicine
    摘要: Recombinant tilapia (Oreochromis mossambicus) fish metallothionein (MT) was used as a surface biosorbent for mercury removal in Escherichia coli. Fish MT conferred better resistance than did mouse or human MT. When tilapia MT (tMT) was fused with an outer-membrane protein, outer membrane protein C (OmpC), the membrane-targeted fusion protein, OmpC-tMT, gave enhanced resistance compared with cytoplasmic tMT expressed in the same host cell. The cytoplasmically expressed tMT showed high mercury adsorption (4.3 ? 0.4?mg/g cell dry weight). The cell surface that expressed E. coli showed about 25% higher adsorption ability (5.6 ? 0.4?mg/g) than the cells expressing cytoplasmic MT, attaining almost twice the level of adsorption of the control plasmid (3.0 ? 0.4?mg/g). As MTs are also known for their ability to scavenge hydroxyl-free radicals, it was also shown that tMT exhibited better radical-scavenging activities than glutathione. These results suggest that fish MT has potential for the development of a bioremediation system for mercury removal that protects the harboring E. coli host by free-radical scavenging.
    日期: 2010-06
    關聯: Applied Microbiology and Biotechnology. 2010 Jun; 87(2):561-569.
    Link to: http://dx.doi.org/10.1007/s00253-010-2466-x
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0175-7598&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000277959500018
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77953083036
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