國家衛生研究院 NHRI:Item 3990099045/4264
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    題名: High prevalence of mutations in quinolone-resistance-determining regions and mtrR loci in polyclonal neisseria gonorrhoeae isolates at a tertiary hospital in southern Taiwan
    作者: Chen, PL;Lee, HC;Yan, JJ;Hsieh, YH;Lee, NY;Ko, NY;Lin, CW;Chang, CM;Wu, CJ;Lee, CC;Ko, WC
    貢獻者: Division of Infectious Diseases
    摘要: Background/Purpose: The emergence of multidrug-resistant Neisseria gonorrhoeae is a great challenge in controlling gonorrhea. This study was conducted to survey the prevalence of molecular mechanisms of antimicrobial resistance among 45 clinical isolates of N. gonorrhoeae collected at a university hospital in Southern Taiwan during 1999-2004. Methods: Mutations in mtrR loci and quinolone-resistance-determining regions (QRDRs) were examined by gene sequencing. Polymerase chain reactions with specific primers were per-formed to detect ermA, ermB, ermC, and ermF. Serogroups and serovars were determined by commercial kits. Results: The percentage of multidrug resistance, that is, resistance to penicillin, tetracycline, erythromycin, and ciprofloxacin, among the 45 isolates was 40%. Ceftriaxone and spectinomycin were active against all isolates in vitro. The frequency of mutations in the QRDR and mtrR promoter was 82.2% and 93.3%, respectively. Eighty-two percent of the isolates carried mutations both in the QRDR and rntrR loci. Of nine mutation profiles with QRDR mutations (n=37), gyrA-Ser91Phe/gyrA-Asp95Gly/parC-Ser87Arg was the most common type (56.8%). Acquired genes for rRNA methylase were detected in 11 isolates (10 ermB and 1 ermA). Twenty-seven serovars were identified and all belonged to serogroup B, which suggested that multiple clones of N. gonorrhoeae were circulating in the community in the Tainan area. Conclusion: The high prevalence of multidrug resistance caused by varied resistance mechanisms in N. gonorrhoeae limits the drug choice. Ongoing surveillance of antimicrobial resistance and discovery of new effective antibiotic therapy are warranted in endemic areas. [J Formos Med Assoc 2010;109(2): 120-127]
    日期: 2010-02
    關聯: Journal of the Formosan Medical Association. 2010 Feb;109(2):120-127.
    Link to: http://dx.doi.org/10.1016/S0929-6646(10)60032-0
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0929-6646&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000275568100005
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77649228907
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